ABSTRACT
The objectives of the present study were to determine Helicobacter pylori via culture, polymerase chain reaction and histopathological diagnosis in 101 children ranging in age from 4 to 18 years, to identify the association among restriction fragment length polymorphism types and clinical disease and to investigate the relationships among different isolates of H. pylori in different age groups. We observed a high prevalence of H. pylori infections in children between the ages of 13 and 18 (75.8%), while children aged 4 to 6 years had the lowest prevalence of infection (40%). H. pylori was detected in 30.7% (31 of 101), 66.3% (67 of 101) and 63.2% (60 of 95) of children as determined by culture methods, PCR and histological examination, respectively. H. pylori isolates with RFLP types I and III were the most common among children with antral nodularity, whereas RFLP types II and IV were the least detected types. Interestingly, all isolates from peptic ulcer patients were type III. Although our results show a high prevalence of H. pylori infections in the pediatric population in eastern Turkey, no association was identified between H. pylori infection with antral nodularity and recurring abdominal pain. In addition, we found low genetic variation among H. pylori isolates from children and no association between RFLP types and antral nodularity (p > 0.05). Additionally, we found that H. pylori isolates with specific RFLP types were predominant in different age groups.
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Genotype , Helicobacter Infections/epidemiology , Helicobacter Infections/microbiology , Helicobacter pylori/classification , Helicobacter pylori/isolation & purification , Molecular Typing , Polymorphism, Restriction Fragment Length , Age Factors , Bacteriological Techniques , Biopsy , Helicobacter pylori/genetics , Molecular Epidemiology , Polymerase Chain Reaction , Prevalence , Turkey/epidemiologyABSTRACT
P300 event-related potentials (ERPs), objective measures related to cognitive processing, have not been studied in Sydenham’s chorea (SC) patients. Purpose: To assess cognitive impairment with P300 ERPs. Method: Seventeen patients with SC and 20 unaffected healthy children were included. Stanford–Binet test was used for psychometric assessment, and odd-ball paradigm was used for auditory ERPs. Results: There was no significant difference in P300 latencies between the SC-pretreatment group, SC-posttreatment group and control group (p>0.05). Mean interpeak latencies in SC-pretreatment group and SC-posttreatment group showed significant prolongation compared with the control group (p<0.05). Mean interpeak latencies in SC-posttreatment group were significantly decreased compared with SC-pretreatment group (p<0.05). Compared to controls, patients did not show significant difference in Stanford-Binet intelligence examination. Conclusion: This report suggests that interpeak latencies and amplitudes of P300 ERPs could be useful for detecting and monitoring cognitive impairment in SC patients. .
Os potenciais evento-relacionados à P300 (ERPs), medidas objetivas relacionadas ao processamento cognitivo, não foram ainda estudados em pacientes com Coreia de Sydenham (CS). Objetivo: avaliar o comprometimento cognitivo através dos ERPs P300. Método: foram incluídos 17 pacientes com CS e 20 crianças saudáveis. A avaliação psicométrica foi feita utilizando o teste de Stanford–Binet e, para os ERPs auditivos, foi usado o paradigma odd-ball. Resultados: Não houve diferença significativa nas latências P300 entre os grupos CS pré-tratamento, CS pós-tratamento e grupo controle. (p>0,05). A média das latências interpicos no grupo CS pré-tratamento e CS pós-tratamento apresentava aumento significativo em comparação aos pacientes do grupo controle (p<0,05). A média das latências interpicos no grupo CS pós-tratamento apresentava decréscimo significativo quando comparada àquela do grupo CS pré-tratamento (p<0,05). Comparados aos controles, os pacientes com CS não mostravam diferença significativa em relação aos controles ao teste de Stanford-Binet. Conclusão: Este estudo sugere que as latências interpicos e as amplitudes dos ERPs P300 podem ser úteis para detectar e monitorar a ocorrência de comprometimento cognitivo em pacientes com CS. .